|
KMID : 0380020100250020215
|
|
Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 2 p.215 ~ p.222
|
|
|
Trends in protein engineering for gene targeting: homing endonucleases and zinc finger nucleases
|
|
Cheong Dea-Eun
Kim Geun-Joong
|
|
|
Abstract
|
|
|
|
Monogenic diseases are resulted from modifications in a single gene of human cells. Because their treatment with pharmacological medicine have a temporary effect, continuous nursing care and retreatment are required. Gene therapy, gene targeting and induced pluripotent stem cell (iPSC) are considered permanent treatment methods of them. In gene therapy, however, retroviral vectors that have potential toxicity caused by random insertion of harmful virus are used as vehicles for transferring genetic materials. On the other hand, gene targeting could replace and remove the modified gene though homologous recombination (HR) induced by site-specific endonucleases. This short reviewprovides a brief overview on the recently tailored endonucleses with high selectivity for HR.
|
|
|
KEYWORD
|
|
|
Gene targeting, homologous recombination, homing endonuclease, zinc finger nuclease
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|